For many of our antibodies, you should aliquot and freeze at -20°C or -80°C. Aliquoting minimizes damage due to freezing and thawing and reduces possible contamination.
Centrifuge the vial at 10,000 x g for 20 seconds—aliquot at least 10 μL into low-protein-binding microcentrifuge tubes. Antibody aliquots should be frozen and thawed only once, with any remainder kept at 4°C.
It is essential to follow the recommendations on the datasheet.