Suggested Buffers

Suggested Buffers for use with Nanoselector Agarose Products:

Buffer

Composition

Lysis Buffer

10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 % NP40, Protease Inhibitor Cocktail and 1 mM PMSF

RIPA Buffer

10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.1 % SDS, 1 % Triton™ X-100, 1% deoxycholate, DNase, MgCl2 (f.c. 2.5 mM), Protease Inhibitor Cocktail and PMSF (f.c. 1 mM)

Dilution Buffer

10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, Protease Inhibitor Cocktail and PMSF

Wash Buffer

10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA

2x SDS-Sample Buffer

120 mM Tris/Cl pH 6.8, 20 % glycerol, 4 % SDS, 0.04 % bromophenol blue, 10 % β-mercaptoethanol

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