Suggested Buffers for use with Nanoselector Agarose Products:
|
Buffer |
Composition |
|
Lysis Buffer |
10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 % NP40, Protease Inhibitor Cocktail and 1 mM PMSF |
|
RIPA Buffer |
10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.1 % SDS, 1 % Triton™ X-100, 1% deoxycholate, DNase, MgCl2 (f.c. 2.5 mM), Protease Inhibitor Cocktail and PMSF (f.c. 1 mM) |
|
Dilution Buffer |
10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, Protease Inhibitor Cocktail and PMSF |
|
Wash Buffer |
10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA |
|
2x SDS-Sample Buffer |
120 mM Tris/Cl pH 6.8, 20 % glycerol, 4 % SDS, 0.04 % bromophenol blue, 10 % β-mercaptoethanol |
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